Macro-simulator for a Lab-on-Chip Demonstration for the use of DNA as a Data Storage System

The goal of this project is to combine fluids with different pH concentrations and predict the solution pH value to represent calculations using DNA substrates. For this purpose, we have designed a multi-stage rotary membrane valve and pump (RMVP) device. The RMVP uses several technologies, including Polydimethylsiloxane (PDMS). This material is used to direct the movement of fluids within our prototype device. The device, which is controlled through a graphical user interface (GUI) connected to a microcontroller unit (MCU), mixes fluids in different chambers. The movement and mixture of fluids in the device represents computations. Based on two fluid inputs of known pH concentrations, we are able to make a prediction of the final fluid mixture pH. This prediction, used as a ground truth, is compared against the final fluid mixture on the output of the device.