Genomics Enterprise Center
Genomics Enterprise Center
The Genomics Enterprise Center at Rochester Institute of Technology employs state-of-the-art genomics technology to produce high-quality data swiftly and affordably.
The Genomics Enterprise Center at Rochester Institute of Technology provides advanced genomics services and resources for faculty, staff, and students. With state-of-the-art equipment and inclusive collaboration, we support a diverse range of research projects. Our dedicated team provides comprehensive training and support services to push the boundaries of genomics. We also welcome external researchers to utilize our world-class facilities and expertise. Let's make groundbreaking discoveries together!
Services and Instrumentation
Our comprehensive services cover Illumina next-generation sequencing and Oxford Nanopore Sequencing. Our laboratory is equipped with Illumina's NovaSeq6000, NextSeq2000, MiSeq, iSeq100, and the Oxford Nanopore MinION. We specialize in library preparation and normalization for RNA-seq, DNA-seq, and whole-genome sequencing. Additionally, we offer DNA/RNA quality control using Qubit, Agilent Technologies Bioanalyzer, and Tapestation systems.
Sample Submission Guidelines
The client must complete a Mandate Sample Submission form provided by the Genomics Enterprise Center. The sample can be delivered in person or shipped to the following address:
Genomics Enterprise Center
Rochester Institute of Technology
Thomas H. Gosnell School of Life Sciences (GOS 1263)
Rochester, NY 14623
USA
Minimum: 100ng of high molecular weight genomic DNA in 50 μl buffer (EB or low TE preferred).
Preferred:2μg of high molecular weight genomic DNA in 100 μl buffer. More is always better.
For Small Numbers of Samples (1-16):
Genomic DNA should be electrophoresed to ensure that the sample is high molecular weight (greater than 10kb in length), and the gel image should be provided to the Genomics Enterprise Center upon submission of the sample. The genomic DNA sample should also have been subjected to Nanodrop spectrophotometry to determine sample concentration and purity. The 260/280 ratio should be close to 1.8, and the 260/230 ratio should be close to 2.0. Nanodrop data concerning sample concentration and purity should be provided to the Center upon submission of the sample. The genomic DNA sample should be resuspended in an appropriate buffer such as TE, Qiagen EB buffer, and water. The volume and identity of the buffer should be provided to the Center upon submission of the sample.
For Large Numbers of Samples (17 and more):
The Genomics Enterprise Center will provide an appropriate 96 well plate for sample submission. The client will array genomic DNA samples in the plate column-wise (i.e., in the order of A1, B1, ..H1, then A2, B2..) and use the well number as the sample number on the Sample Submission Form. At least 20% of the genomic DNA samples should be run out on a gel to ensure that the genomic DNA samples are high molecular weight (greater than 10kb in length). At least 20% of the samples should also be subjected to Nanodrop spectrophotometry to determine sample concentration and purity. The 260/280 ratio should be close to 1.8, and the 260/230 ratio should be close to 2.0. Gel image(s) and Nanodrop data are to be provided to the Center upon submission of the samples. The volume and identity of the buffer should also be provided to the Center upon submission of the samples.
Genomics Enterprise Center Quality Control of DNA Samples
The Genomics Enterprise Center will conduct fluorometric assays (Qubit) to verify sample concentration for each sample. If the samples are not sufficiently concentrated, we may ask the client to submit additional/replacement samples. If the samples are low molecular weight, the client will be asked to provide additional/replacement samples. The Center reserves the right to reject any sample that does not meet internal quality control.
Minimum: 1μg of total RNA in 50ul buffer. Preferred: 4μg of total RNA in 100ul buffer. More is always better. The client may also supply pre-isolated mRNA or Ribominus/RiboZero treated RNA. In both cases, 200 ng of mRNA is required, although more is always better.
For Small Numbers of Samples (1-16):
The RNA sample should be subjected to Nanodrop spectrophotometry to determine sample concentration and purity. The 260/280 and 260/230 ratios should be close to 2.0. Nanodrop data concerning sample concentration and purity should be provided to the Center upon submission of the sample. The total RNA sample should be resuspended in an appropriate buffer such as RNase-free water. The volume and identity of the buffer should be provided to the Center upon submission of the sample.
For Large Numbers of Samples (17 and more):
The Genomics Enterprise Center will provide an appropriate 96 well plate for sample submission. The client will array total RNA samples in the plate column-wise (i.e., in the order of A1, B1, ..H1, then A2, B2..) and use the well number as the sample number on the Sample Submission Form. At least 20% of the samples should also be subjected to Nanodrop spectrophotometry to determine sample concentration and purity. The 260/280 and 260/230 ratios should be close to 2.0. Nanodrop data are to be provided to the Center upon submission of the samples. The volume and identity of the buffer should also be provided to the Center upon submission of the samples.
Quality Control of DNA Samples
The Genomics Enterprise Center will conduct fluorometric assays (Qubit) to verify sample concentration for each sample. The Center will also conduct RNA integrity assays (TapeStation) to determine the integrity of the RNA and determine the RIN value of the samples. If the samples are not sufficiently concentrated, we may ask the client to submit additional/replacement samples. If the samples have low RIN values (an indication of RNA degradation), the Center will ask the client to supply replacement samples if possible. The Center reserves the right to reject any sample that does not meet internal quality control.
Submission of Samples in 96 Well Plate
Submit your samples in the formats shown below in 96 well plate. Start from the left side of the plate and fill-in samples by columns.
Submission Forms
