The iSeq 100 sequencing system stands as Illumina's most approachable, budget-friendly, and swift benchtop sequencer, driven by complementary metal-oxide-semiconductor (CMOS) technology and benefiting from the established accuracy of Illumina's sequencing by synthesis (SBS) chemistry. With the capability to produce 1.2 Gb of data per run in just 17.5 hours, the iSeq 100 System provides the same high resolution and analytical sensitivity as achieved on other Illumina sequencing platforms.